Review



epigallocathecin gallate  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology epigallocathecin gallate
    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM <t>epigallocathecin</t> gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.
    Epigallocathecin Gallate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epigallocathecin gallate/product/Santa Cruz Biotechnology
    Average 93 stars, based on 3 article reviews
    epigallocathecin gallate - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Role of the AMP kinase in cytokine-induced human EndoC-βH1 cell death."

    Article Title: Role of the AMP kinase in cytokine-induced human EndoC-βH1 cell death.

    Journal: Molecular and cellular endocrinology

    doi: 10.1016/j.mce.2015.07.015

    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM epigallocathecin gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.
    Figure Legend Snippet: Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM epigallocathecin gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.

    Techniques Used: Inhibition, Control, Cytometry, Western Blot, Knockdown, Staining, Cell Culture



    Similar Products

    epigallocathecin gallate  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology epigallocathecin gallate
    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM <t>epigallocathecin</t> gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.
    Epigallocathecin Gallate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epigallocathecin gallate/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    epigallocathecin gallate - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    epigallocathecin gallate  (Rooibos Ltd)
    90
    Rooibos Ltd epigallocathecin gallate
    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM <t>epigallocathecin</t> gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.
    Epigallocathecin Gallate, supplied by Rooibos Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/epigallocathecin gallate/product/Rooibos Ltd
    Average 90 stars, based on 1 article reviews
    epigallocathecin gallate - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM epigallocathecin gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.

    Journal: Molecular and cellular endocrinology

    Article Title: Role of the AMP kinase in cytokine-induced human EndoC-βH1 cell death.

    doi: 10.1016/j.mce.2015.07.015

    Figure Lengend Snippet: Fig. 5. Inhibition of STAT-1 does not prevent cytokine-induced EndoC-bH1 cell death. (A) EnodC-bH1 cells were treated with control or STAT-1 siRNA. Two days later cells were exposed to IL-1b þ IFN-g overnight and cell death rates were analyzed by flow cytometry. For immunoblot analysis of P-STAT-1 cells were exposed to cytokines for 30 min. Upper panel shows the means ± SEM for 4 experiments and the lower panel shows a 55% knockdown of Phospho-STAT-1 induced by siRNA treatment. The intensities of the P-STAT-1 bands were normalized to total protein loading and transfer, as assessed by amidoblack staining. (B) and (C) EndoC-bH1 cells were pre-exposed to 10 mM epigallocathecin gallate (EGCG) or 50 ng/ml fludarabine (Flud) for 15 min and then cultured for another 18 h in the presence of IL-1b þ IFN-g before analysis of cell death using propidium iodide staining and flow cytometry. Results are means ± SEM for 4 independent observations.

    Article Snippet: IKK inhibitor X and epigallocathecin gallate was from Santa Cruz.

    Techniques: Inhibition, Control, Cytometry, Western Blot, Knockdown, Staining, Cell Culture